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ci 0035 ht1376 atcc  (ATCC)


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    Structured Review

    ATCC ci 0035 ht1376 atcc
    Ci 0035 Ht1376 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ci+0035+ht1376+atcc/HT-1376/pm37561631-383-195-197
    Average 96 stars, based on 435 article reviews
    ci 0035 ht1376 atcc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    MTT Assay:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Chromatin Immunoprecipitation:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Extraction:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Amplex Red Cholesterol Assay:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Purification:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    DNA Purification:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Real-time Polymerase Chain Reaction:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Western Blot:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    RNA Sequencing:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    shRNA:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023

    Quantitative RT-PCR:

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for qRT-PCR are listed in Method details This paper N/A Recombinant DNA pSIN-EF1a-puro-NFYC-37 This paper N/A pSIN-EF1a-puro-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-37 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D8 exon This paper N/A pSIN-EF1a-puro-SFB-NFYC-50 D10 exon This paper N/A pSIN-EF1a-BSD-NFYC-37 This paper N/A pSIN-EF1a-BSD-NFYC-50 This paper N/A pSIN-EF1a-puro-HA-CBP This paper N/A pSIN-EF1a-puro-HA-p300 This paper N/A pSIN-EF1a-puro-FLAG-nSREBP2 (nuclear form) This paper N/A pSIN-EF1a-puro-CARM1 This paper N/A pSIN-EF1a-puro-V5-CARM1 This paper N/A pSIN-EF1a-puro-HA-CARM1 This paper N/A pcDNA3.1-V5-CARM1 This paper N/A pcDNA3.1-HA-CBP This paper N/A pcDNA3.1-HA-CBP 601R > A This paper N/A pcDNA3.1-HA-CBP 601R > K This paper N/A pcDNA3.1-HA-CBP 672R > A This paper N/A pcDNA3.1-HA-CBP 672R > K This paper N/A pcDNA3.1-FLAG-nSREBP2 (nuclear form) This paper N/A pcDNA3.1 Thermo Fisher Scientific Cat# V79520 pGL3 Luciferase Reporter Vectors Promega Cat# E1751 pLKO.1-puro vector Addgene Cat# 10878 lentiCRISPR-V2-puro Addgene Cat# 52961 Software and algorithms GraphPad Prism v8.0.2 GraphPad software https://www.graphpad.com ImageJ v1.53c National Institutes of Health (NIH) https://ImageJ.nih.gov/ij GSEA Subramanian et al.39 https://www.gsea-msigdb.org/gsea/index.jsp SnapGene v3.2.1 Insightful Science https://www.snapgene.com/ HISAT2 v2.0.5 Kim et al.40 http://daehwankimlab.github.io/hisat2/ Trim Galore v0.6.10 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ BWA v0.7.17 Li et al.41 https://github.com/lh3/bwa MACS2 Zhang et al.42 https://github.com/macs3-project/MACS bedtools v2.28.0 Quinlan and Hall43 https://bedtools.readthedocs.io/en/latest/ IGV v2.16.0 Thorvaldsdóttir et al.44 https://igv.org/ ChIPseeker v1.34.1 Yu et al.45 https://github.com/YuLab-SMU/ChIPseeker ReactomePA v1.42.0 Yu et al.46 https://github.com/YuLab-SMU/ReactomePA deepTools v3.5.2 Ramı́rez et al.47 https://github.com/deeptools/deepTools

    Article Title: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RPMI-1640 GIBCO Cat# C11875500BT FBS ExCell Bio Cat# FSP500 Delipidated FBS TOCYTO Cat# UT04011 ECORI Thermo Fisher Scientific Cat# FD0274 BshTI Thermo Fisher Scientific Cat# FD1464 ESP3I Thermo Fisher Scientific Cat# FD0454 BSP119I Thermo Fisher Scientific Cat# FD0124 KpnI Thermo Fisher Scientific Cat# FD0524 XhoI Thermo Fisher Scientific Cat# FD0694 PrimeStar Max Premix TAKARA Cat# R045A T4 DNA Ligase NEB Cat# M0202L T4 Polynucleotide Kinase NEB Cat# M0201S Biotin Sigma-Aldrich Cat# B4639 Lipofectamine 3000 Invitrogen Cat# L3000015 MTT Beyotime Cat# ST316 Critical commercial assays Chromatin immunoprecipitation (ChIP) kits Cell Signaling Technology Cat# 56383S chloroform-free lipid extraction kit Abcam Cat# ab211044 Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Dual-Luciferase Assay kit Promega Cat# E1910 RNA purification kit TIANGEN Cat# DP419 DNA purification kit TIANGEN Cat# DP214 Genomic DNA kit TIANGEN Cat# DP304 HiScript III RT SuperMix Vazyme Cat# R323 ChamQ SYBR qPCR Master Mix Vazyme Cat# Q331 Western ECL Substrate Tanon Ca# 180-5001 Deposited data RNA-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004546 ChIP-seq data NGDC (https://ngdc.cncb.ac.cn/) Accession code: HRA004618 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 J82 ATCC Cat# HTB-1 5637 ATCC Cat# HTB-9 BIU87 Kunming Cell Bank Cat# CI-0035 HT1376 ATCC Cat# CRL-1472 RT4 ATCC Cat# HTB-2 UMUC3 ATCC Cat# CRL-1749 T24 ATCC Cat# HTB-4 SW780 ATCC Cat# CRL-2169 TCCSUP ATCC Cat# HTB-5 Primary BLCA cell lines (SYBC1) Ma et al.33 N/A Experimental models: Organisms/strains BALB/c nude mice Vital River Laboratory Animal Technology N/A Oligonucleotides shRNA and sgRNA targeting sequences are listed in Method details This paper N/A Primers for ChIP qRT-PCR are listed in Method details This paper N/A (Continued on next page) 16 Cell Reports 42, 112963, August 29, 2023



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